Electrophoretic Mobility Shift Assays for RNA–Protein Complexes
نویسندگان
چکیده
منابع مشابه
Electrophoretic mobility shift assays for RNA-protein complexes.
The electrophoretic mobility shift assay (EMSA), or gel mobility shift assay, is a popular and powerful technique for the detection of RNA-protein interactions. It relies on the fact that naked RNA has certain mobility on nondenaturing gels, but if the RNA is bound by protein, the mobility of the RNA is reduced. Therefore, the binding of protein results in a characteristic upward shift of the R...
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Transcriptional regulation of gene expression is controlled through the binding of sequence-specific DNA-binding proteins (transcription factors) to the regulatory regions of genes. The exact gene expression program of a cell is determined by the spectrum of transcription factors present with the nucleus of a cell. The presence of these factors is dependent upon the cell type being examined and...
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We describe a platform for high-throughput electrophoretic mobility shift assays (EMSAs) for identification and characterization of molecular binding reactions. A photopatterned free-standing polyacrylamide gel array comprised of 8 mm-scale polyacrylamide gel strips acts as a chassis for 96 concurrent EMSAs. The high-throughput EMSAs was employed to assess binding of the Vc2 cyclic-di-GMP ribos...
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thaliana plants. C. R. Acad. Sci. (Paris) 316:1194-1199. 2.Devic, M., S. Albert, M. Delseny, and T.J. Roscoe. 1997. Efficient PCR walking on plant genomic DNA. Plant Physiol. Biochem. 35:331-339. 3.Doyle, J.J. and J. L Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13-15. 4.Frey, M., C. Stettner, and A. Gierl. 1998. General method for gene isolation in tagging approaches: Ampli...
متن کاملElectrophoretic mobility shift assays for the analysis of DNA-protein interactions.
Electromobility shift assay is a simple, efficient, and rapid method for the study of specific DNA-protein interactions. It relies on the reduction in the electrophoretic mobility conferred to a DNA fragment by an interacting protein. The technique is suitable to qualitative, quantitative, and kinetic analyses. It can also be used to analyze conformational changes.
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2014
ISSN: 1940-3402,1559-6095
DOI: 10.1101/pdb.prot080721